MRN-ATMPathwayInhibitor,Mirin-CAS299953-00-7-Calbiochem , 299953-00-7
Synonym(s):
(Z)-5-(4-Hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one, Mirin;MRN-ATM Pathway Inhibitor, Mirin - CAS 299953-00-7 - Calbiochem
CAS NO.：299953-00-7
Empirical Formula: C10H8N2O2S
Molecular Weight: 220.25
MDL number: MFCD05885480
EINECS: 683-709-3
| Pack Size | Price | Stock | Quantity |
| 10MG | RMB1453.94 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | 295-297 °C(Solv: ethanol (64-17-5)) |
| Boiling point: | 441.6±55.0 °C(Predicted) |
| Density | 1.49±0.1 g/cm3(Predicted) |
| storage temp. | 2-8°C |
| solubility | DMSO: >10mg/mL |
| form | Reddish orange powder |
| pka | 9.15±0.30(Predicted) |
| color | red to orange |
| Sensitive | Moisture Sensitive |
| Stability: | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months. |
Description and Uses
Mirin is an inhibitor of the Mre11/Rad50/Nbs1 (MRN) complex, which functions as a DNA damage sensor and activates the DNA repair enzyme ataxia-telangiectasia mutated kinase (ATM). It inhibits phosphorylation of H2AX induced by DNA containing dsDNA breaks (DSBs), indicating decreased ATM activation, with an IC50 value of 66 μM in X. laevis cell-free extracts. Mirin (100 μM) also inhibits the exonuclease activity of Mre11 in a cell-free assay. It induces cell cycle arrest at the G2 phase in TOSA4 cells and inhibits homology-dependent repair in HEK293 cells in a concentration-dependent manner. Nanoparticle-encapsulated mirin (50 mg/kg) reduces tumor growth in an LA-N-5 mouse xenograft model.
Mirin has been used:
- as a small molecule inhibitor of meiotic recombination 11 (MRE11), in the pre-treatment of HCT116 wildtype cells before irradiation
- as Mre11 inhibitior in BSC-1 cells infected with simian virus 40
- in the pre-treatment of W12E cells for quantification of human papilloma virus (HPV) episome levels and to potentiate the effect of polyamide-25 (PA-25)
Safety
| Symbol(GHS) |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P280a-P304+P340-P305+P351+P338-P405-P501a |
| Hazard Codes | Xi |
| Risk Statements | 36 |
| Safety Statements | 26 |
| WGK Germany | 3 |
