Nitrocefin , >90% , 41906-86-9

CAS NO.：41906-86-9

Empirical Formula: C21H16N4O8S2

Molecular Weight: 516.5

MDL number: MFCD00864896

PRODUCT Properties

• Melting point: 103-113° (dec); mp 167-169° (dec) (Lee)
• alpha: D20 -224° (c = 1.0 in dioxane)
• Boiling point: 872.0±65.0 °C(Predicted)
• Density: 1.67±0.1 g/cm3(Predicted)
• storage temp.: 2-8°C
• solubility: DMF: 20 mg/ml; DMSO: 20 mg/ml; DMSO:PBS (pH 7.2) (1:20): 0.04 mg/ml
• form: A crystalline solid
• pka: 2.50±0.50(Predicted)
• color: Yellow to orange
• Stability: Hygroscopic, Unstable in Solution
• InChIKey: LHNIIDJCEODSHA-OQRUQETBSA-N
• SMILES: N12[C@@]([H])([C@H](NC(CC3SC=CC=3)=O)C1=O)SCC(/C=C/C1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O)=C2C(O)=O

Description and Uses

The generation of β-lactamases by bacteria affords resistance to several classes of β-lactam antibiotics, including penicillins and cephalosporins. Nitrocefin is a chromogenic cephalosporin substrate commonly used to detect β-lactamases in bacteria. The presence of β-lactamase activity is indicated by the appearance of a red color that is proportional in intensity to the original concentration of nitrocefin.

Nitrocefin is a chromogenic β-lactamase substrate that undergoes colour change from yellow to red as the amide bond in the β-Lactam ring is hydrolyzed by β-lactamase. Nitrocefin undergoes colour chang es induced by lactamases produced by Gram-positive and Gram-negative bacteria. Several studies have utilized the colour changing properties of Nitrocefin for the detection of β-lactamase activity from bacterial cell extracts by isoelectric focusing and spectroscopy. Nitrocefin has also been used in studies involving β-lactamase resistant antibiotics.

Safety

• Symbol(GHS): GHS02
• Signal word: Warning
• Hazard statements: H228
• Precautionary statements: P210-P260
• HS Code: 29349990