fromseasponge,≥85%(HPLC),waxysolid , 76343-93-6

Synonym(s):
[1R-[1R*, 4Z, 8E, 10Z, 12S*, 15R*, 17R*(R*)]]-4-(17-Hydroxy-5,12-dimethyl-3-oxo-2,16-dioxabicyclo[13.3.1]nonadeca-4,8,10-trien-17-yl)-2-thiazolidinone;LAT-A;Latrunculin A, Latrunculia magnifica - CAS 76343-93-6 - Calbiochem

CAS NO.：76343-93-6

Empirical Formula: C22H31NO5S

Molecular Weight: 421.55

MDL number: MFCD27977552

PRODUCT Properties

• alpha: D24 +152° (c = 1.2 in chloroform)
• storage temp.: -20°C
• solubility: DMSO: soluble
• form: waxy solid
• color: yellow
• optical activity: +144.423 （CHCl3）
• Stability: Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20° for up to 3 months.

Description and Uses

Actin disruption is used to study cell functions in vitro (e.g., migration, endocytosis) and in vivo (e.g., tumor cell invasion). Latrunculin A is a bioactive 2-thiazolidinone macrolide derived from sponges that sequesters G-actin and prevents F-actin assembly. It binds monomeric actin with 1:1 stoichiometry and can be used to block actin polymerization both in vitro (K_d = 0.2 μM) and in cells (0.5 μM, 30 min).{ Latrunculin A (1-10 μM) causes depolymerization of tumor cell cytoskeleton within ten minutes. Overnight treatment of cells with latrunculin A (10 μM) strongly suppresses actin synthesis. Prolonged cell treatment blocks dexamethasone-induced changes in actin cytoskeleton with no effect on cell viability.

Latrunculin A has been used as medium supplementation for A549 cells to determine the internalization mechanism of CAV9 in A549 human lung carcinoma cells.

Safety

• Symbol(GHS): GHS02
• Signal word: Danger
• Hazard statements: H225
• Precautionary statements: P210-P233-P240-P241-P242-P243-P280-P303+P361+P353-P370+P378-P403+P235-P501
• WGK Germany: 3