Methotrexatexhydrate , 98% , 133073-73-1

Synonym(s):
L-4-Amino-N¹⁰-methylpteroylglutamic acid hydrate;L-Amethopterin hydrate;4-Amino-10-methylfolic acid hydrate;Antifolan hydrate;Methylaminopterin hydrate

CAS NO.：133073-73-1

Empirical Formula: C20H22N8O5

Molecular Weight: 454.44

MDL number: MFCD00150847

EINECS: 625-397-3

PRODUCT Properties

• Melting point: 195 °C
• storage temp.: -20°C
• solubility: H₂O: insoluble
• form: powder
• color: yellow
• optical activity: [α]/D +21.0±2.0°
• Stability: Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months.
• InChIKey: FBOZXECLQNJBKD-ZDUSSCGKSA-N

Description and Uses

Methotrexate (MTX) is similar in structure to folic acid and aminopterin . It acts by inhibiting the metabolism of folic acid and blocking key enzymes in the synthesis of purines and pyrimidines required for cell proliferation. MTX is known to induce adenosine release, which mediates many of its anti-inflammatory effects, including the reduction of proinflammatory cytokines. Formulations containing MTX have been used as immunosuppressants, in the treatment of cancer, autoimmune disease, ectopic pregnancy, and for the induction of medical abortions. MTX formulations have been considered the gold standard of disease-modifying antirheumatic drug (DMARD) therapy to treat both the immune-inflammatory and joint destructive processes of rheumatoid arthritis.

Methotrexate hydrate has been used:

• as a dihydrofolate (DHFR)?inhibitor in sphere forming assay culture
• as a component of dulbecco′ modified Eagle′s medium (DMEM) for selection of transfected cells
• in viability and co-culture assays

Safety

• Symbol(GHS): GHS06,GHS08
• Signal word: Danger
• Hazard statements: H301-H315-H319-H360FD
• Precautionary statements: P201-P202-P264-P301+P310-P302+P352-P305+P351+P338
• Hazard Codes: T
• Risk Statements: 61-25-36/38-46
• Safety Statements: 53-26-36/37-45-36
• RIDADR: UN 2811 6.1/PG 3
• WGK Germany: 3
• RTECS: MA1225000
• HS Code: 29339980