E-64 , Proteinase inhibitor, 99% , 66701-25-5

Synonym(s):
E-64;N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide;n-[n-( l-transepoxysuccinic acid)-l-leucyl]-agmatine;n-[n-(l-3-trans-carboxirane-2-carbonyl)-l-leucyl]-4-aminobutylguanidine;L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatine

CAS NO.：66701-25-5

Empirical Formula: C15H27N5O5

Molecular Weight: 357.41

MDL number: MFCD07366493

EINECS: 613-978-4

PRODUCT Properties

• Melting point: >170°C (dec.)
• Density: 1.44±0.1 g/cm3(Predicted)
• storage temp.: 2-8°C
• solubility: H₂O: 20 mg/mL, clear, colorless
• pka: 3.16±0.40(Predicted)
• form: White solid
• color: White or off-white
• Water Solubility: Soluble in DMSO (25mg/ml), or water (20mg/ml)
• Sensitive: Light Sensitive
• BRN: 1405664
• Stability: Stable for 1 year from date of purchase as supplied.
• InChIKey: LTLYEAJONXGNFG-DCAQKATOSA-N
• SMILES: O1[C@H](C(N[C@H](C(NCCCCNC(N)=N)=O)CC(C)C)=O)[C@H]1C(O)=O

Description and Uses

E-64 (66701-25-5) is a cell-permeable, epoxysuccinyl peptide irreversible inhibitor of calpain and other cysteine proteases.1,2?Inhibits calpain-dependent apoptosis pathway in T cells3?but promotes heat-induced apoptosis in FM3A cells. Typical working concentration is 0.5-10 μg/ml.4

It is used as a broad spectrum cysteine proteinase and calpain activation inhibitor. Additionally, Prevotella intermediate studies report that E-64 blocks the breakdown of methaemoglobin and the oxidation of oxyhaemoglobin by InpA. E-64 has also been used to study excystation in Giardia lamblia. E-64 is an inhibitor of Calpain, cathepsin K and cathepsin S. E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained

Safety

• Symbol(GHS): GHS08
• Signal word: Warning
• Hazard statements: H371
• Precautionary statements: P260-P264-P270-P308+P311-P405-P501
• Safety Statements: 22-24/25
• WGK Germany: 3
• RTECS: RR0390000
• F: 10
• HS Code: 29252900