Pentafluorobenzenesulfonylfluorescein , ≥98% , 728912-45-6

CAS NO.：728912-45-6

Empirical Formula: C26H11F5O7S

Molecular Weight: 562.42

MDL number: MFCD08062167

PRODUCT Properties

• storage temp.: Store at -20°C
• solubility: DMF: 30 mg/ml; DMSO: 30 mg/ml; DMSO:PBS (pH 7.2)(1:1): .25 mg/ml; Ethanol: 20 mg/ml
• form: A crystalline solid
• color: Light yellow to yellow

Description and Uses

Reactive oxygen species (ROS) play important roles in the initiation and progression of many disease processes. Most fluorescent probes for ROS detection, such as 2,7-dichlorofluorescein (DCFH), function by an oxidative mechanism and are useful for total oxidant detection but are not selective for hydrogen peroxide specifically.¹ Pentafluorobenzenesulfonyl fluorescein is a fluorescent probe that functions by a nonoxidative mechanism. It fluoresces upon perhydrolysis of the sulfonyl linkage, and is selective for hydrogen peroxide over hydroxyl radical, tert-butyl hydroperoxide, superoxide anion, singlet oxygen, and nitrates. Pentafluorobenzenesulfonyl fluorescein can be used as a probe for hydrogen peroxide in whole cell systems.¹

Pentafluorobenzenesulfonyl fluorescein is a H2O2-selective sensor that can be used to detect H2O2 levels in cells. Pentafluorobenzenesulfonyl fluorescein is normally non-fluorescent but fluoresces upon perhydrolysis of the sulfonyl linkage by H2O2[1].