Succinimidyl3-[3-[2-(biotinamido)ethyl]amino-3-oxopropyl]dithio]propionate , >90% , 142439-92-7

Synonym(s):
(2-[Biotinamido]ethylamido)-3,3′-dithiodipropionic acid N-hydroxysuccinimide ester

CAS NO.：142439-92-7

Empirical Formula: C22H33N5O7S3

Molecular Weight: 575.72

MDL number: MFCD00466919

PRODUCT Properties

• Melting point: 129-131°C
• Density: 1.43
• storage temp.: −20°C
• solubility: DMF: 50 mg/mL
• pka: 13.90±0.40(Predicted)
• form: powder

Description and Uses

Biotin-bisamido-SS-NHS is a cleavable reagent which can efficiently introduce a biotin moiety to amine-containing biomolecules. The cross-bridge and extended spacer arm are helpful in minimizing steric hindrance involved with the binding to avidin or streptavidin probes. This biotin compound is membrane-permeable and useful for intracellular labeling. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP to remove the biotin label.

NHS SS Biotin is a amine reactive biotinylation reagent that labels antibodies and other proteins and molecules that contain primary amines. NHS SS Biotin reacts efficiently with pirmary amino groups in alkaline buffers to form stable amide bonds. The disulfide bond in the spacer arm of NHS SS Biotin is also cleavable using reducing agents such as TCEP and DTT.

Safety

• Symbol(GHS): GHS08
• Signal word: Danger
• Hazard statements: H360D
• Precautionary statements: P201-P202-P280-P308+P313-P405-P501
• Hazard Codes: T
• Risk Statements: 61
• Safety Statements: 53-23-36/37/39-45
• WGK Germany: 1
• HS Code: 29349990