Home Categories 2',7'-Dichlorodihydrofluoresceindiacetate
A7083358

2',7'-Dichlorodihydrofluoresceindiacetate , 10mMinDMSO , 4091-99-0

Synonym(s):
2ʹ,7ʹ-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem;H₂DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate

CAS NO.:4091-99-0

Empirical Formula: C24H16Cl2O7

Molecular Weight: 487.29

MDL number: MFCD00128955

EINECS: 685-843-8

Pack Size Price Stock Quantity
1ml RMB159.20 In Stock
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Update time: 2022-07-08

PRODUCT Properties

Melting point: 209-210 °C(lit.)
Boiling point: 593.5±50.0 °C(Predicted)
Density  1.463±0.06 g/cm3(Predicted)
storage temp.  -20°C
solubility  DMF: soluble
pka 3.79±0.36(Predicted)
form  Crystalline solid
color  White to off-white
BRN  4610273
Stability: Light Sensitive
EPA Substance Registry System 2',7'-Dichlorohydrofluorescein diacetate (4091-99-0)

Description and Uses

DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M−1cm−1).

2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.

Safety

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319
Precautionary statements  P501-P270-P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313-P301+P312+P330
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  22-24/25-26
WGK Germany  3
10-21
HS Code  29329990

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