3-(N-Morpholino)-2-hydroxy-1-propanesulfonic acid , 99% , 68399-77-9

Synonym(s):
β-Hydroxy-4-morpholinepropanesulfonic acid;3-Morpholino-2-hydroxypropanesulfonic acid;MOPSO

CAS NO.：68399-77-9

Empirical Formula: C7H15NO5S

Molecular Weight: 225.26

MDL number: MFCD00009629

EINECS: 269-989-6

PRODUCT Properties

• Melting point: 275-280 °C (dec.)
• Density: 1.416±0.06 g/cm3(Predicted)
• storage temp.: Sealed in dry,Room Temperature
• solubility: H₂O: 0.5 M at 20 °C, clear
• pka: pK1:6.75 (37°C)
• form: Crystalline Powder
• color: White
• PH Range: 6.2 - 7.6
• Odor: Odorless
• Water Solubility: Water solubility under desired conditions ca.112,6 g/L at 20°C.
• BRN: 1109697
• InChI: InChI=1S/C7H15NO5S/c9-7(6-14(10,11)12)5-8-1-3-13-4-2-8/h7,9H,1-6H2,(H,10,11,12)
• InChIKey: NUFBIAUZAMHTSP-UHFFFAOYSA-N
• SMILES: C(N1CCOCC1)C(O)CS(O)(=O)=O
• CAS DataBase Reference: 68399-77-9(CAS DataBase Reference)
• EPA Substance Registry System: 4-Morpholinepropanesulfonic acid, .beta.-hydroxy- (68399-77-9)

Description and Uses

MOPSO Free Acid is a zwitterionic aminosulfonate buffer with a similar structure to MOPS and a useful pH range of 6.5 - 7.9. It may be used as a buffer component in various biological applications including DNA gel electrophoresis. It has been found that MOPSO buffer combined excellent pH stabilization at pH 7·00 and limited color alteration by testing the color changes of raw sugar solutions so that MOPSO buffer and three glass-fiber filters in series can be used to reduce the effect of pH and turbidity on color measurement of raw sugar solution[1]. The activity of the endo-α-D-mannosidase enzyme in MOPSO buffers at pH 7.0 is increased very much over buffers like HEPES or HEPPS. This buffer also has been employed as a buffer component of charcoal yeast extract medium[2]. However, morpholine family buffers (MES, MOPS, and MOPSO) have been reported to interact with the trivalent metal ions Cr³⁺ and Fe³⁺. Therefore, extensive testing is required before using MAPSO.

MOPSO is a biological buffer also referred to as a second generation “Good′s” buffer which displays improved solubility compared to traditional “Good′s” buffers. The pKa of MOPSO is 6.9 which make it an ideal candidate for buffer formulations which require a pH slightly below physiological to maintain a stable environment in solution. MOPSO is considered to be non-toxic to culture cell lines and provides high-solution clarity.

MOPSO can be used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H315-H319-H335
• Precautionary statements: P261-P264-P271-P280-P302+P352-P305+P351+P338
• Hazard Codes: Xi
• Risk Statements: 36/37/38
• Safety Statements: 26-36-37/39
• WGK Germany: 1
• TSCA: Yes
• HS Code: 29349990