K252b , ≥97%(HPLC) , 99570-78-2

CAS NO.：99570-78-2

Empirical Formula: C26H19N3O5

Molecular Weight: 453.45

MDL number: MFCD09878299

EINECS: 999-999-2

PRODUCT Properties

• Boiling point: 769.8±60.0 °C(Predicted)
• Density: 1.78±0.1 g/cm3(Predicted)
• storage temp.: −20°C
• solubility: Soluble in DMSO (up to 1 mg/ml) or in DMF (up to 1 mg/ml).
• form: ready-to-use solution
• pka: 3.33±0.40(Predicted)
• color: White or off-white
• Stability: Stable for 1 year from date of purchase as supplied. Solutions in DMSO or DMF may be stored at -20° for up to 3 months.

Description and Uses

K252b is an indolocarbazole isolated from the actinomycete Nocardiopsis, first described as an inhibitor of protein kinase C. However, as this compound does not freely pass through the cell membrane, it is used to inhibit extracellular kinases (ectokinases) of cells in culture. K252b inhibits receptor-mediated degranulation from basophil-like RBL-2H3 cells (IC₅₀ = 0.5 μg/ml) and human basophils. This extracellular inhibitor is also used in comparison studies with the closely related, cell-permeable inhibitor K252a, particularly in studies of neuronal differentiation.

K252A is a staurosporine analogue isolated from a Nocardiopsis strain as a potent inhibitor of protein kinase C. K252A exhibits potent antitumour activity but shows no antimicrobial activity in vitro, or in vivo toxicity in rodents. While K252A is a potent inhibitor of Ca2+/calmodulin kinase II, it is also active against other kinases, notably myosin light chain kinase, cAMP-dependent protein kinase (PKA), protein kinase C (PKC) and cGMP-dependent protein kinase (PKG).

Safety

• Hazard Codes: Xi
• RIDADR: UN 1993 / PGIII
• HS Code: 29349990