JC-1 , 95% , 3520-43-2

Synonym(s):
5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide;5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide;CBIC2(3)

CAS NO.：3520-43-2

Empirical Formula: C25H27Cl4N4.I

Molecular Weight: 652.228

MDL number: MFCD00188475

EINECS: 200-258-5

PRODUCT Properties

• Melting point: 275–278 ℃
• storage temp.: room temp
• solubility: Soluble in methanol, N,N-dimethyl formamide, dimethyl sulfoxide
• form: crystals
• color: Deep red
• λmax: 514nm
• Stability: Store as supplied desiccated at -20°C for up to 2 years from the date of purchase. Solutions in DMSO or distilled water may be stored at 4°C for up to 1 year. DO NOT FREEZE/THAW SOLUTIONS. Sterilize solutions only by filtration, not by autoclaving
• Biological Applications: Detectingmitochondrialmembrane potential,ABCB1,ABCC1,and ABCG2 transporters inhibitors,nucleic acid hybridization,prostate cancer; treating cellular death,Alzheimer’s disease; apoptosis assay; cytotoxicity assay; hematotoxicity
• Major Application: Langmuir-Blodgett films; lasing systems; nonlinear optical materials; photographic materials1,
• InChIKey: FYNNIUVBDKICAX-UHFFFAOYSA-M

Description and Uses

JC-1 is a membrane-permeable cationic dye that is used to study mitochondrial integrity in the context of cellular apoptosis. It selectively enters mitochondria and changes fluorescence characteristics with alteration in mitochondrial transmembrane potential (ΔΨm). In healthy cells with a high mitochondrial ΔΨm, JC-1 forms complexes known as J-aggregates, which fluoresce red/orange and may be detected using FL2 settings by flow cytometry. A drop in ΔΨm, a very early event in apoptosis, results in JC-1 monomers, which fluoresce green (FL1 settings on flow cytometers). JC-1 may also be used with fluorescent microscopes or plate readers, using excitation at 520-570 nm and emission at 570-610 nm for J-aggregates and excitation at 485 nm and emission at 535 nm for monomers.

A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent "J-aggregates," which exhibit broad exc

Safety

• Symbol(GHS): GHS07,GHS09
• Signal word: Warning
• Hazard statements: H315-H410-H335-H319-H302
• Precautionary statements: P273-P391-P501-P264-P280-P302+P352-P321-P332+P313-P362-P264-P270-P301+P312-P330-P501-P264-P280-P305+P351+P338-P337+P313P
• WGK Germany: 3
• F: 10-21