Nile Red , Used for fluorescence analysis, ≥95.0%(HPLC) , 7385-67-3
Synonym(s):
Nile Blue A Oxazone
CAS NO.：7385-67-3
Empirical Formula: C20H18N2O2
Molecular Weight: 318.37
MDL number: MFCD00011639
EINECS: 230-966-0
| Pack Size | Price | Stock | Quantity |
| 250MG | RMB220.80 | In Stock |
|
| 1G | RMB636.00 | In Stock |
|
| 5G | RMB2463.20 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | 203-205 °C(lit.) |
| Boiling point: | 457.53°C (rough estimate) |
| Density | 1.1871 (rough estimate) |
| refractive index | 1.5700 (estimate) |
| storage temp. | 2-8°C |
| solubility | methanol: soluble1mg/mL |
| form | Powder |
| pka | 4.08±0.20(Predicted) |
| color | Dark red to maroon |
| BRN | 279110 |
| Stability: | Stable. Incompatible with strong oxidizing agents. |
| InChI | InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3 |
| InChIKey | VOFUROIFQGPCGE-UHFFFAOYSA-N |
| SMILES | C12=CC=CC=C1C(=O)C=C1C2=NC2=C(C=C(N(CC)CC)C=C2)O1 |
Description and Uses
Nile red is a dye based on the benzo[a]phenoxazine core. Nile Red (9-diethylbenzo[a]phenoxazin-5- one) stands out as electrically neutral and water-insoluble since it features a carbonyl group at the 5-position that readily accepts the electron density. This makes it a strong push?pull chromophore, which underlies its distinct solvatochromicity and ability to indicate, e.g., cellular events in live-cell imaging. In addition, Nile Red’s inherent lipophilicity, near-infrared (NIR) emission, large Stokes shift, two-photon absorptivity (2PA), and low background fluorescence (as the luminosity is quenched in aqueous (aq) media) render it a near-ideal histochemical stain for studying membranes and lipid droplets in cells. The increase of its exquisite solvent sensitivity and steep emission in hydrophobic environments has also been employed in various forms of stochastic super-resolution localization microscopy[1].
Nile Red is a photostable, lipophilic stain with bright red fluorescence. Dyes and metabolites.
Safety
| Symbol(GHS) |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302-H315-H319-H335 |
| Precautionary statements | P261-P280-P301+P312-P302+P352-P305+P351+P338 |
| Safety Statements | 24/25 |
| WGK Germany | 3 |
| F | 8-10 |
| HS Code | 29349990 |
