Iodonitrotetrazolium chloride , 98% , 146-68-9

Synonym(s):
p-Iodonitrotetrazolium Violet;2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride;INT

CAS NO.：146-68-9

Empirical Formula: C19H13ClIN5O2

Molecular Weight: 505.7

MDL number: MFCD00011961

EINECS: 205-676-2

PRODUCT Properties

• Melting point: 240 °C (dec.)(lit.)
• storage temp.: 2-8°C
• solubility: methanol: water (1:1): 50 mg/mL hot, very faintly turbid, very deep yellow
• form: Powder, Crystals and/or Chunks
• color: White to off-white
• Water Solubility: soluble
• Sensitive: Light Sensitive
• λmax: 248nm
• BRN: 4093224
• Stability: Stable. Incompatible with strong oxidizing agents.
• Biological Applications: Bacterial vaginosis diagnosis assay; dehydrogenase enzyme assay; diagnostic assay; food and beverage analytes assays; microbial growth assay; detecting bacteria,yeast,fungi,gamma-hydroxybutyric acid (GHB),microbial growth; measuring niacin,bacterial respiratory activity,superoxide dismutase;treating cancer
• InChI: InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
• InChIKey: JORABGDXCIBAFL-UHFFFAOYSA-M
• SMILES: [N+]1(=NC(C2C=CC=CC=2)=NN1C1C=CC(I)=CC=1)C1=CC=C([N+]([O-])=O)C=C1.[Cl-]

Description and Uses

Iodonitrotetrazolium (INT) (chloride) is a monotetrazolium salt used as an indicator dye. It is reduced to an insoluble formazan that is used as a vital dye or indicator of cellular redox activity. Reduction commonly results from the activity of dehydrogenases, although non-enzymatic electron transfer reactions can occur in the presence of an intermediate electron acceptor. INT is commonly used to measure the respiratory activity of microorganisms in a variety of contexts.

Iodonitrotetrazolium chloride (INT) on reduction gets converted to iodonitrotetrazolium formazan, a water insoluble violet colored dye or indicator. Hence INT can be used for the colorimetric assay of various dehydrogenases.

Safety

• Symbol(GHS): GHS02,GHS07
• Signal word: Danger
• Hazard statements: H228-H335
• Precautionary statements: P210-P240-P241-P261-P271-P280
• Hazard Codes: Xn,F,Xi
• Risk Statements: 20/21/22-68/20/21/22-36/37/38-11
• Safety Statements: 36/37-36/37/39-26-22-16
• RIDADR: 2811
• WGK Germany: 3
• F: 3-8-10
• TSCA: Yes
• HazardClass: 6.1
• PackingGroup: III
• HS Code: 29339900