CoomassiebrilliantblueG-250 , 10mMinDMSO , 6104-58-1

Synonym(s):
Brilliant Blue G;Acid blue 90;Coomassie Brilliant Blue G;Cyanine G;Polar Blue G

CAS NO.：6104-58-1

Empirical Formula: C47H48N3NaO7S2

Molecular Weight: 854.02

MDL number: MFCD00078482

EINECS: 228-058-4

PRODUCT Properties

• Melting point: 100 °C
• Density: 1.274[at 20℃]
• vapor pressure: 0.093Pa at 20℃
• Flash point: 11 °C
• storage temp.: 2-8°C
• solubility: H2O: soluble1mg/mL
• form: Solid
• Colour Index: 42655
• color: Dark blue-violet-brown
• Water Solubility: Soluble in water (50 mg/ml) and ethanol (40 mg/ml).
• BRN: 5230822
• Stability: Light Sensitive
• InChIKey: JFNOCRSXNFZMNT-UHFFFAOYSA-M
• LogP: -2.55 at 20℃

Description and Uses

Brilliant Blue G is used in analytical biochemistry dye and as a P2X7 antagonist. It is also used in protein staining following gel electrophoresis. It is used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery. It is useful for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualized during staining. It has been used in the Bradford dye-binding protein assay. A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H315-H319-H335
• Precautionary statements: P271-P280
• WGK Germany: 3
• TSCA: Yes
• HS Code: 32041200