2',7'-Dichlorofluorescein , Indicator, ≥90.0% , 76-54-0

Synonym(s):
2′,7′-Dichlorofluorescein

Update time: 2022-07-08

Melting point:	280 °C (dec.)(lit.)
Boiling point:	514.77°C (rough estimate)
Density	0.79 g/mL at 20 °C
refractive index	1.5300 (estimate)
Flash point:	12°C (53.6°F)
storage temp.	room temp
solubility	alcohol: passes test
pka	4.46(at 25℃)
form	crystalline
color	orange to red-brown, powder
PH Range	4.0 (colorless) - 6.6 (green)
Water Solubility	Soluble in ethanol and methanol. Slightly dimethyl sulfoxide. Insoluble in water.
λmax	509nm
BRN	58009
Stability:	Stable. Incompatible with strong oxidizing agents.
Major Application	Electroluminescent devices, sol-gel silica films, integrated circuits, photoresists, lithographic printing plates, recording material, LED devices, photoconductors, Solid-state dye laser, lithographic printing plates, inks, Fluorescent pH sensors, emissive probes, chemotherapeutic treatment of disease, phototherapeutic treatment of disease, photodynamic treatment of disease
InChIKey	NZVMJBGGAQSNLI-UHFFFAOYSA-N
CAS DataBase Reference	76-54-0(CAS DataBase Reference)
EPA Substance Registry System	Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',7'-dichloro-3',6'-dihydroxy- (76-54-0)

2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.
A new fluorescent sensor for Cd2+ and its application in living cells imaging. Tetrahedron Lett. 2015, 56 (11), 1322-1327.
Colorimetric and fluorogenic signaling of fluoride ions by thiophosphinated dichlorofluorescein. Dyes Pigm. 2015, 112, 170-175.