4-Chloro-7-nitro-1,2,3-benzoxadiazole , 98% , 10199-89-0

Synonym(s):
4-Chloro-7-nitro-1,2,3-benzoxadiazole;NBD-chloride

CAS NO.：10199-89-0

Empirical Formula: C6H2ClN3O3

Molecular Weight: 199.55

MDL number: MFCD00005808

EINECS: 233-496-4

PRODUCT Properties

• Melting point: 97-99 °C(lit.)
• Boiling point: 333.1±45.0 °C(Predicted)
• Density: 2.0589 (rough estimate)
• refractive index: 1.5810 (estimate)
• storage temp.: Inert atmosphere,Room Temperature
• solubility: Soluble in methanol, dimethylsulfoxide, dimethylformamide and chloroform.
• pka: -5.65±0.50(Predicted)
• form: Crystalline Powder
• color: Yellow to light brown
• Water Solubility: soluble
• BRN: 614212
• CAS DataBase Reference: 10199-89-0(CAS DataBase Reference)
• EPA Substance Registry System: 2,1,3-Benzoxadiazole, 4-chloro-7-nitro- (10199-89-0)

Description and Uses

4-Chloro-7-nitrobenzofurazan is used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines. It is utilized in the preparation of fluorescent phospholipid-derivative, hydroxynaphthofurazan and 4-chloro-7-nitrobenzofurazan- didecanoylphosphatidylethanolamine. It serves as a fluorescent reagent to label free sulfhydryls and N-terminals within proteins and trapping agent for cysteine sulfenic acid. It is a sensitive chromogenic and fluorogenic reagent. It reacts with trans-1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene (Danishefsky's diene) to prepare regioselectively the silyl enol ether. Further, it is used to label peptides, proteins and other biomolecules. In addition it is used in the synthesis of fluorescent phospholipid-derivative, NBD-didecanoylphosphatidylethanolamine, functionalized hydroxynaphthofurazan and 7-nitrobenzofurazan (NBD)-labeled maleimide.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H315-H319
• Precautionary statements: P302+P352-P305+P351+P338
• Hazard Codes: Xn,Xi
• Risk Statements: 22-36/38
• Safety Statements: 36/37/39
• RIDADR: UN 2811 6.1/PG 3
• WGK Germany: 3
• RTECS: DF8002400
• F: 10-21
• TSCA: Yes
• HS Code: 29349990