5(6)-Carboxyfluorescein, mixture of isomers , ≥95%(HPLC), 5-and 6-heterogeneous mixture , 72088-94-9

Synonym(s):
5(6)-Carboxyfluorescein;5(6)-FAM;5,6-CF

CAS NO.：72088-94-9

Empirical Formula: C42H24O14

Molecular Weight: 752.63

MDL number: MFCD00151081

EINECS: 276-331-1

PRODUCT Properties

• Melting point: 275 °C
• Boiling point: 464.9°C (rough estimate)
• Density: 1.3418 (rough estimate)
• refractive index: 1.6380 (estimate)
• storage temp.: Keep in dark place,Inert atmosphere,Room temperature
• solubility: DMSO (Sparingly), Methanol (Slightly, Heated)
• form: Solid
• color: Light Yellow to Dark Brown
• Water Solubility: slightly soluble
• InChIKey: CPRVUBZYHZGASQ-UHFFFAOYSA-N
• SMILES: C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1

Description and Uses

5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.

As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H319
• Precautionary statements: P305+P351+P338
• Hazard Codes: Xi,Xn
• Risk Statements: 36-36/37/38-20/21/22
• Safety Statements: 37/39-26-36-22
• WGK Germany: 3
• TSCA: Yes
• HS Code: 29322090