Home Categories Chemical Reagents 5-FITC
A1581350

5-FITC , ≥95%(HPLC) , 3326-32-7

Synonym(s):
FITC;Fluorescein isothiocyanate;Fluorescein 5-isothiocyanate;FITC, Isomer I;Fluorescein Isothiocyanate, Isomer I

CAS NO.:3326-32-7

Empirical Formula: C21H11NO5S

Molecular Weight: 389.38

MDL number: MFCD00005063

EINECS: 222-042-0

Pack Size Price Stock Quantity
100mg RMB79.20 In Stock
250mg RMB159.20 In Stock
1g RMB472.00 In Stock
5g RMB2220.80 In Stock
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Update time: 2022-07-08

PRODUCT Properties

Melting point: >360 °C(lit.)
Boiling point: 708.6±60.0 °C(Predicted)
Density  1.3423 (rough estimate)
refractive index  1.5060 (estimate)
storage temp.  2-8°C
solubility  Slightly soluble in DMSO, ethanol, methanol DMF and acetone
form  powder
pka 2.2, 4.4, 6.7(at 25℃)
color  White
PH Range Weak green uorescence (6.0) to strong green uorescence (7.2)
Water Solubility  practically insoluble
Sensitive  Moisture Sensitive
λmax 490nm
BRN  1407295
Stability: Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids.
Major Application Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia
Biological Applications Analyzing sugar chain; detecting iodide/iodate or total iodine; detecting/imaging thiols; detecting/quantifying biotin derivatives;16 diagnosing classical Hodgkin lymphoma (CHL) in lymph nodes; cellulose nanocrystals for bioimaging applications; treating neuroinflammation; treating/diagnosing cancer; PEO-PCL-PEO triblock copolymers for topical delivery; polymersomes in biomedical or cosmetic applications;apoptosis assay; as a substrate for measuring heparanase activity, human immunodeficiency virus (HIV-1) proteinase activity, kinase activity, phosphatase activity, β-lactamase activity, transglutaminase (TG2) activity; implantable drug-delivery devicess
InChIKey MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description and Uses

Fluorescein 5-isothiocyanate (5-FITC) is an isomer of fluorescein isothiocyanate (FITC), which is commonly used as a mixture of the 5- and 6-isothiocyanate isomers. It reacts with amine and thiol groups to form conjugates with proteins, lipids, and other molecules for detection by a variety of fluorescent-based applications. 5-FITC displays excitation/emission maxima of 494/519 nm, respectively.

Fluorescein isothiocyanate isomer I has been used for the staining of conidia. It is a kind of fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines and it is also used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.

Safety

Symbol(GHS) 
GHS08
Signal word  Danger
Hazard statements  H317-H334
Precautionary statements  P261-P272-P280-P284-P302+P352-P333+P313
Hazard Codes  Xn,Xi
Risk Statements  42/43-36/37/38-22
Safety Statements  22-45-36/37/39-26-36/37-36
WGK Germany  3
10
TSCA  Yes
HS Code  29329990

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