Fluorescein5(6)-isothiocyanate , 95%, 5-and 6-heterogeneous mixture , 27072-45-3

CAS NO.：27072-45-3

Empirical Formula: C21H11NO5S

Molecular Weight: 389.38

MDL number: MFCD00005063

EINECS: 248-207-7

PRODUCT Properties

• Melting point: >360 °C(lit.)
• storage temp.: -20°C
• solubility: DMSO (Slightly), Methanol (Slightly)
• form: powder
• color: Brown to Dark Brown
• Merck: 14,4089
• BRN: 1407295
• Stability: Moisture Sensitive
• InChIKey: WBSWSUYBEIMVMI-UHFFFAOYSA-N

Description and Uses

Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
Excitation source: 488 nm spectral line, argon-ion laser
Excitation wavelength: 494 nm
Emission wavelength: 518 nm
Extinction coefficient: > 70,000 M-1cm-1
Solubility: Soluble in aqueous buffers at pH > 6
Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0

Fluorescein Isothiocyanate is a fluorescent reagent with incompletely defined position of the NCS. Fluorescein Isothiocyanate is used in multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance, fluorescence imaging. and drug delivery. Dyes and metabolites.

Safety

• Symbol(GHS): GHS07,GHS08
• Signal word: Danger
• Hazard statements: H334-H302+H332-H315-H317-H319-H335
• Precautionary statements: P264-P270-P271-P272-P280-P284-P301+P312+P330-P302+P352+P333+P313+P363-P304+P340+P312-P305+P351+P338+P337+P313-P403+P233-P405-P501-P261-P342+P311
• Hazard Codes: Xn
• Risk Statements: 42/43-36/37/38-20/21/22-42
• Safety Statements: 22-36-26-24/25-45-36/37
• WGK Germany: 3
• F: 10
• HS Code: 29321900