2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymet , 1mg/mLinDMSO , 117464-70-7

Synonym(s):
2ʹ,7ʹ- bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester;2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester;BCECF/AM - CAS 117464-70-7 - Calbiochem;BCECF-acetoxymethyl

CAS NO.：117464-70-7

Empirical Formula: C42H40O21

Molecular Weight: 880.75

MDL number: MFCD00036969

PRODUCT Properties

• Boiling point: 692.41°C (rough estimate)
• Density: 1.2837 (rough estimate)
• refractive index: 1.6500 (estimate)
• Flash point: 85 °C
• storage temp.: -20°C
• form: White to off-white solid
• color: Orange to red

Description and Uses

BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.

Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H319-H315-H335
• Precautionary statements: P264-P280-P302+P352-P321-P332+P313-P362-P264-P280-P305+P351+P338-P337+P313P
• Hazard Codes: Xi
• Risk Statements: 36/37/38
• Safety Statements: 22-26-36
• WGK Germany: 3