5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt , Used for molecular biology, ≥99% , 6578-06-9
Synonym(s):
5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt;BCIP p-toluidine salt;X-phosphate p-toluidine salt
CAS NO.：6578-06-9
Empirical Formula: C8H6BrClNO4P.C7H9N
Molecular Weight: 433.63
MDL number: MFCD00040636
EINECS: 229-506-1
| Pack Size | Price | Stock | Quantity |
| 100MG | RMB159.20 | In Stock |
|
| 500MG | RMB559.20 | In Stock |
|
| 1g | RMB919.20 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | 194-195℃ |
| storage temp. | -20°C |
| solubility | DMF: 20 mg/mL |
| form | tablets |
| color | White to Orange to Green |
| InChIKey | QEIFSLUFHRCVQL-UHFFFAOYSA-N |
| CAS DataBase Reference | 6578-06-9(CAS DataBase Reference) |
| EPA Substance Registry System | 1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9) |
Description and Uses
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Safety
| Symbol(GHS) |    GHS02,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H226-H312+H332-H319-H360D |
| Precautionary statements | P201-P210-P261-P280-P308+P313-P370+P378 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38-40 |
| Safety Statements | 26 |
| RIDADR | 2811 |
| WGK Germany | 3 |
| F | 3-8-10 |
| HazardClass | 6.1 |
| PackingGroup | III |
| HS Code | 29339190 |
