5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt , ≥99%(HPLC) , 6578-06-9

Synonym(s):
5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt;BCIP p-toluidine salt;X-phosphate p-toluidine salt

CAS NO.：6578-06-9

Empirical Formula: C8H6BrClNO4P.C7H9N

Molecular Weight: 433.63

MDL number: MFCD00040636

EINECS: 229-506-1

PRODUCT Properties

• Melting point: 194-195℃
• storage temp.: -20°C
• solubility: DMF: 20 mg/mL
• form: tablets
• color: White to Orange to Green
• InChIKey: QEIFSLUFHRCVQL-UHFFFAOYSA-N
• CAS DataBase Reference: 6578-06-9(CAS DataBase Reference)
• EPA Substance Registry System: 1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9)

Description and Uses

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Safety

• Symbol(GHS): GHS02,GHS07,GHS08
• Signal word: Danger
• Hazard statements: H226-H312+H332-H319-H360D
• Precautionary statements: P201-P210-P261-P280-P308+P313-P370+P378
• Hazard Codes: Xi
• Risk Statements: 36/37/38-40
• Safety Statements: 26
• RIDADR: 2811
• WGK Germany: 3
• F: 3-8-10
• HazardClass: 6.1
• PackingGroup: III
• HS Code: 29339190