Brefeldin A , fromPenicilliumbrefeldianum,≥98%(HPLC) , 20350-15-6

Synonym(s):
BFA;1,6,7,8,9,11a,12,13,14,14a-Decahydro-1,13-dihydroxy-6-methyl-4H-cyclopent[f]oxacyclotridecin-4-one;Ascotoxin;γ,4-Dihydroxy-2-(6-hydroxy-1-heptenyl)-4-cyclopentanecrotonic acid λ-lactone;Cyanein

CAS NO.：20350-15-6

Empirical Formula: C16H24O4

Molecular Weight: 280.36

MDL number: MFCD00083258

EINECS: 606-528-3

PRODUCT Properties

• Melting point: 200-205 °C
• Boiling point: 492.7±45.0 °C(Predicted)
• alpha: 93 º (C=2 IN MEOH)
• Density: 1.108±0.06 g/cm3(Predicted)
• vapor pressure: 0.56 hPa ( 20 °C)
• Flash point: 87 °C
• storage temp.: Sealed in dry,Store in freezer, under -20°C
• solubility: methanol: 10 mg/mL, clear, colorless to faintly yellow
• pka: 12.92±0.60(Predicted)
• form: Crystalline Powder
• color: White to almost white
• Water Solubility: Soluble in dimethylsulfoxide, dichloromethane and ethanol. Slightly soluble in water.
• Merck: 13,1355
• BRN: 25191
• Stability: Stable for 2 years from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.
• InChIKey: QSECOIOBNZLGOD-OBCVZTHZSA-N
• CAS DataBase Reference: 20350-15-6

Description and Uses

Brefeldin A (20350-15-6) is a specific inhibitor of protein translocation from endoplasmic reticulum to Golgi. Induces apoptosis. Brefeldin A activates sphingomyelin metabolism. Cell permeable.

Brefeldin A (BFA) is a natural fungal metabolite which has been used extensively to study intracellular transport by vesicles or endosomes. Early studies demonstrated that BFA reversibly interferes with protein trafficking and secretion mediated by the Golgi apparatus and endoplasmic reticulum. BFA directly and reversibly inhibits Sec7 domain-containing guanine-exchange factors which are necessary for ADP-ribosylation factor activation associated with vesicular transport (IC50 = ~10 μM). BFA is used to study endosomal trafficking and function in cells of plants as well as those of fungi, invertebrates, and vertebrates.

Safety

• Symbol(GHS): GHS06
• Signal word: Danger
• Hazard statements: H301
• Precautionary statements: P301+P310
• Hazard Codes: Xn,T
• Risk Statements: 22-25-36/37/38-20/21/22
• Safety Statements: 24/25-45-36-26
• RIDADR: UN 2811 6.1/PG 3
• WGK Germany: 3
• RTECS: GY8410000
• Autoignition Temperature: 301 °C
• HS Code: 29411090
• Toxicity: LD50 i.p. in mice: >200 mg/kg (Haerri)