formicroscopyCertistain , 17372-87-1

Synonym(s):
Lysine

CAS NO.：17372-87-1

Empirical Formula:

Molecular Weight:

MDL number:

Description and Uses

Optimal coating concentrations must be determined by end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.

1. Thaw Poly-L-Lysine solution at room temperature.
2. Dilute Poly-L-Lysine solution to desired concentration in sterile water.
3. Fully coat the cell culture surface with diluted Poly-L-Lysine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.
4. Allow cell culture vessel to sit at room temperature overnight.
5. Aspirate the Poly-L-Lysine solution the following day and rinse vessel with sterile water followed by coating with desired ECM protein.