Epidermal Growth Factor from murine submaxillary gland , EGF,suitableforcellculture , 62229-50-9

Synonym(s):
EGF;Epidermal Growth Factor;Epidermal Growth Factor, Human, Recombinant, E. coli;EGF, Human, Recombinant, E. coli;EGF, Murine Submaxillary Glands, Receptor Grade

CAS NO.：62229-50-9

Empirical Formula: C257H381N73O83S7

Molecular Weight: 6045.67

MDL number: MFCD00132565

EINECS: 263-468-7

PRODUCT Properties

• storage temp.: -20°C
• form: powder
• color: white to off-white

Description and Uses

Epidermal growth factor (EGF) is a polypeptide ligand for the EGF receptor (EGFR; ). It is synthesized as a 1,217 amino acid glycosylated transmembrane precursor protein from which mature, soluble EGF is formed by proteolysis. Mature EGF is a 53-amino acid polypeptide that contains six cysteine residues, which form three intramolecular disulfide bonds essential to the biological activity of EGF. EGF is synthesized in the brain, kidney, pancreas, small intestine, pituitary gland, and submaxillary gland and is found in various bodily fluids, including saliva, cerebrospinal fluid, blood, breast milk, and prostatic fluid. Binding of EGF to the EGFR induces receptor dimerization, autophosphorylation, and intracellular signaling with roles in cell proliferation. In patients with early Parkinson’s disease and Alzheimer’s disease with amnestic mild cognitive impairment, low plasma EGF levels are predictive of future long-term cognitive decline. Plasma levels of EGF are reduced in pregnant woman infected with T. cruzi compared with uninfected pregnant controls. Exogenous application of EGF normalizes cortical GABAergic transmission and improves rotarod performance in the R6/2 transgenic mouse model of Huntington''s disease. Probiotic administration of E. coli engineered to secrete human EGF induces epithelial barrier restoration in a mouse model of intestinal ulcers. Immunization with human EGF induces an anti-EGF antibody response and increased survival in mice with EGFR-expressing tumors. Cayman''s EGF (human, recombinant) protein can be used for cell-based assay applications. This protein consists of 54 amino acids and has a calculated molecular mass of 6.3 kDa.

Epidermal Growth Factor (EGF) from murine submaxillary gland has been used as a mitogen, triggering the cell to commence mitosis, in a variety of cell lines. In tissue cultures, EGF acts to reduce or eliminate the requirement for serum and can be used in conjunction with other media additives and hormone. The working concentration for EGF is generally 0.1-10 ng/mL.

Safety

• Hazard Codes: B
• WGK Germany: 3
• RTECS: KB4518000
• F: 10-21