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S8975914

Forhybridization , 68938-01-2

CAS NO.:68938-01-2

Empirical Formula: C17H15BrFNO3

Molecular Weight: 380.208

MDL number: MFCD00130921

EINECS: 309-566-6

Pack Size Price Stock Quantity
1ml RMB344.42 In Stock
5x1ml RMB1725.74 In Stock
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Update time: 2022-07-08

PRODUCT Properties

Density  1.00 g/mL at 20 °C
storage temp.  -20°C
solubility  50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow
form  buffered aqueous solution
color  Off-white
EPA Substance Registry System Deoxyribonucleic acids, salmon sperm (68938-01-2)

Description and Uses

Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.

Safety

Hazard Codes  B
Safety Statements  22-24/25
WGK Germany  3
RTECS  HG1933000
3-10-21
TSCA  Yes
HS Code  29349990

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