HEPES , 10mMinWater , 7365-45-9

Synonym(s):
HEPES;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid;HEPES solution;N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid);N-2-Hydroxyethylpiperazine-N?-2-ethanesulfonic Acid

CAS NO.：7365-45-9

Empirical Formula: C8H18N2O4S

Molecular Weight: 238.3

MDL number: MFCD00006158

EINECS: 230-907-9

PRODUCT Properties

• Melting point: 234-238 °C
• Boiling point: 408℃[at 101 325 Pa]
• Density: 1.07 g/mL at 20 °C
• vapor pressure: 0Pa at 25℃
• refractive index: n20/D 1.339
• storage temp.: 2-8°C
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• form: powder
• color: White
• PH: 5.0-6.5 (1Maqueous solution)
• pka: 7.5(at 25℃)
• PH Range: 7.0 - 7.6
• Water Solubility: Soluble
• λmax: λ: 260 nm Amax: 0.06; λ: 280 nm Amax: 0.04
• Merck: 14,4654
• BRN: 883043
• Stability: Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
• InChIKey: JKMHFZQWWAIEOD-UHFFFAOYSA-N
• LogP: -3.85 at 20℃

Description and Uses

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H302-H315-H319-H335
• Precautionary statements: P261-P271-P280
• Hazard Codes: Xi
• Risk Statements: 36/37/38
• Safety Statements: 24/25-22-36-26
• WGK Germany: 1
• RTECS: TL6809000
• TSCA: Yes
• HS Code: 29335995