Tris(hydroxymethyl)aminomethane hydrochloride , Used for molecular biology and cell culture, ≥99.0%(AT) , 1185-53-1
Synonym(s):
TRIS HCl;TRIS hydrochloride;Trizma hydrochloride;Tris(hydroxymethyl)aminomethane hydrochloride;tris(Hydroxymethyl)aminomethane, HCl
CAS NO.：1185-53-1
Empirical Formula: C4H12ClNO3
Molecular Weight: 157.6
MDL number: MFCD00012590
EINECS: 214-684-5
| Pack Size | Price | Stock | Quantity |
| 100G | RMB460.00 | In Stock |
|
| 500G | RMB1099.20 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | 150-152 °C |
| Boiling point: | 225℃[at 101 325 Pa] |
| Density | 1.05 g/mL at 20 °C |
| vapor pressure | 0Pa at 20℃ |
| storage temp. | room temp |
| solubility | H2O: 4 M at 20 °C, clear, colorless |
| form | crystalline |
| color | clear colorless (40 % (w/w) in H2O) solution |
| PH | 2.5-4.0 (25℃, 4M in H2O) |
| pka | 8.1(at 25℃) |
| PH Range | 7.0 - 9.0 |
| Water Solubility | SOLUBLE |
| Sensitive | Hygroscopic |
| λmax | λ: 260 nm Amax: 0.12 λ: 280 nm Amax: 0.08 |
| BRN | 3675235 |
| Stability: | Stable. Incompatible with strong oxidizing agents, bases, moisture. |
| InChIKey | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| LogP | -3.6 at 20℃ |
Description and Uses
Abbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0. The compound is instrumental in preparing Laemmli buffer, one of the most widely used SDS-PAGE buffers, and also finds application in various custom running and loading buffers, frequently in combination with glycine and SDS. Lysis, the initial step of DNA extraction, involves a buffer containing Tris and EDTA (ethylenediaminetetraacetic acid). In this process, EDTA binds to divalent cations such as calcium and magnesium. Due to their role in maintaining cell membrane integrity, their removal by EDTA leads to membrane destabilization. Tris plays a key role as the primary buffering component, maintaining the pH of the buffer at a stable point, typically around 8.0. Additionally, Tris may interact with the LPS (lipopolysaccharide) in the membrane, contributing to further membrane destabilization. This underscores Tris HCl's significance in various laboratory procedures.
Tris Hydrochloride is a buffering medium for electrophoresis, molecular biology, and cell culture applications. It may be combined with Tris Base to simplify Tris buffer preparation. Tris Hydrochloride has been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. In immunohistochemistry, Tris Hydrochloride has also been used with urea as a method for antigen retrieval.
Safety
| Symbol(GHS) |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P305+P351+P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | 3 |
| RTECS | TY2900000 |
| F | 3 |
| TSCA | Yes |
| HS Code | 29221980 |
