Tris(hydroxymethyl)aminomethane hydrochloride , Reagent level, ≥99% , 1185-53-1

Synonym(s):
TRIS HCl;TRIS hydrochloride;Trizma hydrochloride;Tris(hydroxymethyl)aminomethane hydrochloride;tris(Hydroxymethyl)aminomethane, HCl

CAS NO.：1185-53-1

Empirical Formula: C4H12ClNO3

Molecular Weight: 157.6

MDL number: MFCD00012590

EINECS: 214-684-5

PRODUCT Properties

• Melting point: 150-152 °C
• Boiling point: 225℃[at 101 325 Pa]
• Density: 1.05 g/mL at 20 °C
• vapor pressure: 0Pa at 20℃
• storage temp.: room temp
• solubility: H₂O: 4 M at 20 °C, clear, colorless
• form: crystalline
• color: clear colorless (40 % (w/w) in H₂O) solution
• PH: 2.5-4.0 (25℃, 4M in H2O)
• pka: 8.1(at 25℃)
• PH Range: 7.0 - 9.0
• Water Solubility: SOLUBLE
• Sensitive: Hygroscopic
• λmax: λ: 260 nm Amax: 0.12; λ: 280 nm Amax: 0.08
• BRN: 3675235
• Stability: Stable. Incompatible with strong oxidizing agents, bases, moisture.
• InChIKey: QKNYBSVHEMOAJP-UHFFFAOYSA-N
• LogP: -3.6 at 20℃

Description and Uses

Abbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0.

Tris Hydrochloride is a buffering medium for electrophoresis, molecular biology, and cell culture applications. It may be combined with Tris Base to simplify Tris buffer preparation. Tris Hydrochloride has been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. In immunohistochemistry, Tris Hydrochloride has also been used with urea as a method for antigen retrieval.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H315-H319-H335
• Precautionary statements: P305+P351+P338
• Hazard Codes: Xi
• Risk Statements: 36/37/38
• Safety Statements: 26-36
• WGK Germany: 3
• RTECS: TY2900000
• F: 3
• TSCA: Yes
• HS Code: 29221980