PR-619 , 10mMinDMSO , 2645-32-1

Synonym(s):
2,6-Diamino-3,5-dithiocyanopyridine;Thiocyanic acid C,C′-(2,6-diamino-3,5-pyridinediyl) ester; 2,6-Diaminopyridine-3,5-bis(thiocyanate)

CAS NO.：2645-32-1

Empirical Formula: C7H5N5S2

Molecular Weight: 223.28

MDL number: MFCD00830384

EINECS: 200-256-5

PRODUCT Properties

• Melting point: 210℃
• Boiling point: 406.0±45.0 °C(Predicted)
• Density: 1.61±0.1 g/cm3(Predicted)
• storage temp.: -20°C
• solubility: DMSO: soluble5mg/mL (clear solution)
• pka: -0.19±0.50(Predicted)
• form: powder
• color: white to beige
• Stability: Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 month.

Description and Uses

PR-619 (2645-32-1) is a nonselective, reversible inhibitor of cysteine-reactive deubiquitinating enzymes (DUBs; 5-20 μM)1. PR-619 is highly useful for preserving ubiquitinated proteins during cell lysis. DUBs become unregulated in cell lysates and will act quickly to deubiquitinate proteins of interest during cell lysis. To preserve maximum amounts of ubiquitinated protein, include PR-619 (50-100 μM) in lysis buffers. PR-619 is also useful for interrogating the role of DUBs in intact cell systems2, and treatment of cells results in an overall accumulation of ubiquitinated proteins and many other phenotypes.

PR-619 has been used:

• as a component of lysis buffer and also as a deubiquitinase inhibitor to treat proteins derived from SILAC-labeled Jurkat cells
• as a pan-deubiquitinase inhibitor to study its effect on adenoassociated virus (AAV) transduction
• in radioimmunoprecipitation assay buffer (RIPA) buffer used for ubiquitination assays

Safety

• Symbol(GHS): GHS05,GHS07
• Signal word: Danger
• Hazard statements: H302-H315-H317-H318-H335
• Precautionary statements: P261-P280-P305+P351+P338
• Hazard Codes: Xn
• Risk Statements: 22-37/38-41-43
• Safety Statements: 26-36/37/39
• WGK Germany: 3