Home Categories Biochemical Engineering PR-619
A7637158

PR-619 , 10mMinDMSO , 2645-32-1

Synonym(s):
2,6-Diamino-3,5-dithiocyanopyridine;Thiocyanic acid C,C′-(2,6-diamino-3,5-pyridinediyl) ester; 2,6-Diaminopyridine-3,5-bis(thiocyanate)

CAS NO.:2645-32-1

Empirical Formula: C7H5N5S2

Molecular Weight: 223.28

MDL number: MFCD00830384

EINECS: 200-256-5

Pack Size Price Stock Quantity
1ml RMB399.20 In Stock
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Update time: 2022-07-08

PRODUCT Properties

Melting point: 210℃
Boiling point: 406.0±45.0 °C(Predicted)
Density  1.61±0.1 g/cm3(Predicted)
storage temp.  -20°C
solubility  DMSO: soluble5mg/mL (clear solution)
pka -0.19±0.50(Predicted)
form  powder
color  white to beige
Stability: Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 month.

Description and Uses

PR-619 (2645-32-1) is a nonselective, reversible inhibitor of cysteine-reactive deubiquitinating enzymes (DUBs; 5-20 μM)1. PR-619 is highly useful for preserving ubiquitinated proteins during cell lysis. DUBs become unregulated in cell lysates and will act quickly to deubiquitinate proteins of interest during cell lysis. To preserve maximum amounts of ubiquitinated protein, include PR-619 (50-100 μM) in lysis buffers. PR-619 is also useful for interrogating the role of DUBs in intact cell systems2, and treatment of cells results in an overall accumulation of ubiquitinated proteins and many other phenotypes.

PR-619 has been used:

  • as a component of lysis buffer and also as a deubiquitinase inhibitor to treat proteins derived from SILAC-labeled Jurkat cells
  • as a pan-deubiquitinase inhibitor to study its effect on adenoassociated virus (AAV) transduction
  • in radioimmunoprecipitation assay buffer (RIPA) buffer used for ubiquitination assays

Safety

Symbol(GHS) 
GHS05,GHS07
Signal word  Danger
Hazard statements  H302-H315-H317-H318-H335
Precautionary statements  P261-P280-P305+P351+P338
Hazard Codes  Xn
Risk Statements  22-37/38-41-43
Safety Statements  26-36/37/39
WGK Germany  3

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