Tricine , 99.5% , 5704-04-1

Synonym(s):
Tricine;Tricine, N-Tris(hydroxymethyl)methylglycine;Tris Buffer substance;Tris(hydroxymethyl)aminomethane Buffer substance;Tris(hydroxymethyl)aminomethane hydrochloride buffer solution

CAS NO.：5704-04-1

Empirical Formula: C6H13NO5

Molecular Weight: 179.17

MDL number: MFCD00004277

EINECS: 227-193-6

PRODUCT Properties

• Melting point: 186-188 °C (dec.)
• Boiling point: 311.69°C (rough estimate)
• Density: 1.05 g/mL at 20 °C
• vapor pressure: 0.002-0.004Pa at 20-25℃
• refractive index: 1.4240 (estimate)
• storage temp.: Keep in dark place,Inert atmosphere,Room temperature
• solubility: H₂O: 4 M at 20 °C, clear, colorless
• form: crystalline
• color: clear colorless (40 % (w/w) in H₂O) solution
• PH: 4.0-6.0 (1M in H2O)
• pka: 8.1(at 25℃)
• PH Range: 7.4 - 8.8
• Odor: Odorless
• Water Solubility: soluble
• Merck: 14,9651
• BRN: 1937804
• Stability: Stable.
• LogP: -2.87 at 25℃
• CAS DataBase Reference: 5704-04-1(CAS DataBase Reference)
• EPA Substance Registry System: Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)

Description and Uses

Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets. It is a zwitterionic amino acid commonly useful in the pH range of 7.4-8.8 and the pKa is 8.05 at 25°C. This buffer is also used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H320
• Precautionary statements: P403-P501c
• Hazard Codes: Xi
• Risk Statements: 36/37/38
• Safety Statements: 26-36-36/37/39
• WGK Germany: 3
• F: 3
• TSCA: Yes
• HS Code: 29052900