bisBenzimideH33342trihydrochloride , 10mMinDMSO , 23491-52-3

Synonym(s):
;2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride;bisBenzimide

CAS NO.：23491-52-3

Empirical Formula: C27H28N6O

Molecular Weight: 452.55

MDL number: MFCD00166996

EINECS: 245-691-1

PRODUCT Properties

• Boiling point: 725.9±70.0 °C(Predicted)
• Density: 1.274±0.06 g/cm3(Predicted)
• storage temp.: 2-8°C
• solubility: H2O: soluble20mg/mL
• form: Yellow solid
• pka: 10.97±0.10(Predicted)
• color: Brown to breen
• Water Solubility: Water : 1 mg/mL (2.21 mM; ultrasonic and warming and heat to 80°C)
• BRN: 1234011

Description and Uses

Hoechst 33342 is an A:T-specific DNA minor groove ligand that is a widely used fluorochrome for visualizing cellular DNA. Excitation of the dye occurs around 350 nm with an emission maxima around 461 nm. Hoechst-33342, unlike other Hoechst dyes, is more lipophillic due to an additional ethyl group. The compound has been observed to displace several known DNA intercalators. Adriamycin has been shown to disrupt the DNA dye update. The compound can be used in sorting living cells based on DNA content by FACS, in the visualization of chromatin distribution in living cells, to detect BrdU incorporation into cells, the visualization of fixed cells by microscopy, and to study the initial stages of apoptosis and cell-cycle distribution. In addition to Hoechst-33342''s fluorescent capability, the

Safety

• Symbol(GHS): GHS07,GHS08
• Signal word: Warning
• Hazard statements: H319-H302-H315-H335-H341
• Precautionary statements: P280-P301+P312+P330-P261-P280a-P304+P340-P305+P351+P338-P405-P501a
• Hazard Codes: Xn,C
• Risk Statements: 22-37/38-36/37/38-20/21/22-37-35
• Safety Statements: 22-36/37-24/25-36-26-45-36/37/39
• WGK Germany: 3
• RTECS: DT4200000
• F: 3-8-10
• HS Code: 32129000
• Toxicity: dni-hmn-hla 300 nmol/L CRNGDP 13,2389,1992