3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT) , 10mMinDMSO , 298-93-1
Synonym(s):
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue;Methylthiazolyldiphenyl-tetrazolium bromide;MTT;Thiazolyl blue, MTT
CAS NO.：298-93-1
Empirical Formula: C18H16BrN5S
Molecular Weight: 414.32
MDL number: MFCD00011964
EINECS: 206-069-5
| Pack Size | Price | Stock | Quantity |
| 1ml | RMB159.20 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | 195 °C (dec.) (lit.) |
| Density | 1.4886 (estimate) |
| storage temp. | 2-8°C |
| solubility | methanol: 5 mg/mL, clear |
| form | powder |
| color | Yellow to orange |
| Water Solubility | Slightly soluble.Clear yellow solution at 5mg in 1ml of water on vortexing |
| λmax | 378 nm, 242 nm |
| Sensitive | Light Sensitive |
| BRN | 3825277 |
| Stability: | Hygroscopic |
| Biological Applications | Cell viability assay; microbial growth assays; DNA quantification assays; tissue viability assays; detecting enzymes; measuring membrane potential; treating Alzheimer’s disease,asthma,cancer |
| InChIKey | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
| CAS DataBase Reference | 298-93-1(CAS DataBase Reference) |
| EPA Substance Registry System | 2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (298-93-1) |
Description and Uses
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Safety
| Symbol(GHS) |   GHS07,GHS08 |
| Signal word | Warning |
| Hazard statements | H315-H319-H335-H341 |
| Precautionary statements | P201-P202-P261-P302+P352-P305+P351+P338-P308+P313 |
| Hazard Codes | Xi,T,Xn |
| Risk Statements | 46-68-36/37/38 |
| Safety Statements | 22-24/25-53-45-36/37-26 |
| WGK Germany | 3 |
| RTECS | XF8060000 |
| F | 3-8-10 |
| TSCA | Yes |
| HS Code | 29341000 |
| Hazardous Substances Data | 298-93-1(Hazardous Substances Data) |
