PRODUCT Properties
| Melting point: | 133 °C(lit.) |
| Boiling point: | 300 °C(lit.) |
| Density | 1.261 g/cm3 |
| Flash point: | >230 °F |
| storage temp. | 2-8°C |
| solubility | DMF: 10 mg/ml; DMSO: 10 mg/ml; DMSO:PBS (pH 7.2) (1:2): 0.33 mg/ml |
| form | powder to crystal |
| pka | 15.10±0.70(Predicted) |
| color | White to Light yellow |
Description and Uses
Cinobufagin is a cardiotonic steroid that has been found in the skin of toads of genus Bufo and has diverse biological activities. It inhibits Na+/K+-ATPase activity in guinea pig heart ventricular muscle homogenates by 45% when used at a concentration of 0.3 μM. Cinobufagin is cytotoxic to HCT116 colorectal cancer cells in vitro with IC50 values of less than 50 ng/ml at 48- and 72-hour time points and induces apoptosis in a concentration-dependent manner. In vivo, cinobufagin (10 mg/kg) reduces tumor growth in an HCT116 mouse xenograft model. Cinobufagin (1 μg/ml) inhibits LPS-induced expression of MHC class II, CD80, and CD86 and release of IL-6, IL-8, TNF-α, and IL-10 in human monocyte-derived dendritic cells. It also increases expression of the antimicrobial peptides hBD2 and hBD3 in dendritic cells. Cinobufagin exhibits dose-dependent antinociceptive effects in the hot-plate, acetic acid writhing, and formalin tests in mice.
Cinobufagine is a major component of cinobufacini (huachansu), is an important cardenolidal steroid. Cinobufagine was shown to exhibit potent anti-cancer effects.
Safety
| Symbol(GHS) |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300-H310-H330 |
| Precautionary statements | P260-P264-P280-P284-P302+P350-P310 |
| Hazard Codes | Xi,T+,Xn |
| Risk Statements | 36/37/38-26/27/28-42/43-20/22 |
| Safety Statements | 26-36-45-36/37/39-22-24/25 |
| RIDADR | UN 2811 6.1/PG 1 |
| WGK Germany | 3 |
| RTECS | GD7850000 |
| HazardClass | 6.1(a) |
| PackingGroup | II |
| HS Code | 29322090 |
