Propidium iodide , 94% , 25535-16-4

Synonym(s):
3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide;3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide;3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium, diiodide;Propidium iodide solution

CAS NO.：25535-16-4

Empirical Formula: C27H34IN4+

Molecular Weight: 541.5

MDL number: MFCD00011921

EINECS: 247-081-0

PRODUCT Properties

• Melting point: 220-225 °C (dec.) (lit.)
• Density: 1.5072 (estimate)
• storage temp.: 2-8°C
• solubility: Difficult to mix.
• form: Flaky Powder
• color: Dark red
• Water Solubility: Soluble in water at 1mg/ml
• Sensitive: Light Sensitive & Hygroscopic
• λmax: 493 nm, 535 nm
• BRN: 3843838
• Biological Applications: Detecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens,cancer
• Major Application: Electroluminescent displays; photoresists

Description and Uses

Propidium iodide (PI) is membrane impermeant and generally excluded from viable cells. However, it can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. It binds to DNA and RNA by intercalating between the bases. PI is excited at 488-535 nm with an emission maximum of 617 nm. This probe is suitable for fluorescence microscopy and flow cytometry.

Used as fluorescent stain for nucleic acids,and used in flow cytometry to evaluate cell viability

Safety

• Symbol(GHS): GHS08
• Signal word: Warning
• Hazard statements: H341
• Precautionary statements: P201-P202-P280-P308+P313-P405-P501
• Hazard Codes: Xi,Xn
• Risk Statements: 36/37/38-68-67-48/20/22-40-36/38-22-20-63
• Safety Statements: 26-36-22-36/37
• WGK Germany: 3
• RTECS: SF7949600
• F: 3-8-10-23
• HS Code: 29339900