4-Nitrophenyl butyrate , ≥98% , 2635-84-9
Synonym(s):
p-Nitrophenyl butyrate;Butyric acid 4-nitrophenyl ester
CAS NO.:2635-84-9
Empirical Formula: C10H11NO4
Molecular Weight: 209.2
MDL number: MFCD00047734
EINECS: 258-258-3
Pack Size | Price | Stock | Quantity |
1G | RMB116.00 | In Stock |
|
5G | RMB382.40 | In Stock |
|
25G | RMB1358.40 | In Stock |
|
others | Enquire |
PRODUCT Properties
Boiling point: | 184-185 °C |
Density | 1.19 g/mL at 20 °C(lit.) |
storage temp. | -20°C |
solubility | chloroform: 0.1 g/mL, clear, faintly yellow |
form | A neat oil |
color | Colorless to light yellow |
BRN | 1879729 |
InChIKey | DVDUMIQZEUTAGK-UHFFFAOYSA-N |
Description and Uses
P-nitrophenyl butyrate also kown as 4-nitrophenyl butyrate, is a probe for lipase activity. It is hydrolyzed by lipase in the water ambient condition, giving rise to p-nitrophenol and butyric acid. P-nitrophenol actually occurs in the anionic form, p-nitrophenolate, giving a yellowish coloration and absorbing light at 410 nm in an alkaline buffer such as Tris-HCl pH 7.5.
4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.