Home Categories Analytical Chemistry Ninhydrin
A6159312

Ninhydrin , AR , 485-47-2

Synonym(s):
1,2,3-Indantrione monohydrate;2,2-Dihydroxy-1,3-dioxohydrindene, 2,2-Dihydroxy-1,3-indandione, Indantrione hydrate, Triketohydrindene hydrate;2,2-Dihydroxy-1,3-indanedione;Ninhydrin;Trioxohydrindene monohydrate

CAS NO.:485-47-2

Empirical Formula: C9H6O4

Molecular Weight: 178.14

MDL number: MFCD00003791

EINECS: 207-618-1

Pack Size Price Stock Quantity
5G RMB42.40 In Stock
25G RMB164.00 In Stock
100G RMB574.40 In Stock
500G RMB2880.80 In Stock
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Update time: 2022-07-08

PRODUCT Properties

Melting point: 250 °C (dec.) (lit.)
Boiling point: 230.35°C (rough estimate)
Density  0.862
vapor density  6.16 (vs air)
refractive index  1.4209 (estimate)
Flash point: 13 °C
storage temp.  Keep in dark place,Inert atmosphere,Room temperature
solubility  Soluble in water (50 mg/ml,with sonication), ethanol (100 mg/ml), chloroform (Slightly), ether (Slightly), and methanol (100 mg/ml).
pka 8.47±0.20(Predicted)
form  Powder/Solid
Specific Gravity 0.788
color  white to brownish-white, crystals
PH 4.6-5.0 (10g/l, H2O, 20℃)
Odor Odorless
PH Range 4.6 - 5.0
Water Solubility  0.1-0.5 g/100 mL at 20 ºC
Sensitive  Light Sensitive
Merck  14,6554
BRN  1910963
Stability: Stable. Incompatible with amines, alkalies, strong bases. Light-sensitive.
InChIKey FEMOMIGRRWSMCU-UHFFFAOYSA-N
LogP 0.670

Description and Uses

Ninhydrin is historically the most common reagent for processing fingerprints on porous substrates. It has been used as a fingerprint reagent since the 1950s but has been used as a cell stain since the early 1900s. Ninhydrin molecules form a complex with amino acids resulting in a purple product known as Ruhmann's purple. Purple fingerprints are easily visible on light-colored porous substrates but may be difficult to see on patterned or dark-colored substrates. DFO reacts with amino acids to produce a fluorescent product that is not readily seen with the naked eye. This makes it a useful reagent on dark or patterned porous items. DFO prints are viewed with an alternate light source or laser set to 530–570 nm (green light) through a red barrier filter. 1,2-indanedione is an emerging fingerprint reagent that results in visible pink fingerprints that are also fluorescent. The fluorescence is viewed under the same conditions as DFO.
These chemicals can be used on their own or in sequence to develop latent prints on porous substrates. If they are used in sequence, they are used in the following order: indanedione, DFO, ninhydrin. If a reagent is used out of sequence, it will inhibit or limit the effectiveness of the other reagents. In this laboratory exercise you will develop fingerprints on a porous substrate using indanedione, DFO, and ninhydrin.

ninhydrin structure

Ninhydrin is used for the detection of free amino groups in amino acids, peptides and proteins.

Safety

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319
Precautionary statements  P301+P312+P330-P302+P352-P305+P351+P338
Hazard Codes  Xn,T,F
Risk Statements  22-36/37/38-39/23/24/25-20/21/22-11
Safety Statements  26-28A-45-16-36
RIDADR  UN 1170 3/PG 2
WGK Germany  3
RTECS  NK5425000
8
TSCA  Yes
HazardClass  3
PackingGroup  II
HS Code  29144000
Toxicity LD50 orally in Rabbit: 600 mg/kg

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