Nitrotetrazolium Blue chloride , Molecular biology grade, 98% , 298-83-9

Synonym(s):
NBT;Nitrotetrazolium Blue chloride;Nitroblue tetrazolium chloride;NBT, Nitro BT;Nitro BT

CAS NO.：298-83-9

Empirical Formula: C40H30ClN10O6+

Molecular Weight: 782.19

MDL number: MFCD00012159

EINECS: 206-067-4

PRODUCT Properties

• Melting point: 200°C
• Density: 1.5521 (rough estimate)
• refractive index: 1.7350 (estimate)
• storage temp.: 2-8°C
• solubility: methanol: 50 mg/mL, clear, very strongly yellow
• form: Powder/Solid
• color: Yellow
• Water Solubility: Soluble in water, ethanol and methanol.
• Sensitive: Light Sensitive
• λmax: 256 nm
• BRN: 4115923
• Stability: Stable, but store cold.
• Biological Applications: Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting
• EPA Substance Registry System: 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride (298-83-9)

Description and Uses

Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.

Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.

Safety

• Symbol(GHS): GHS07,GHS08
• Signal word: Danger
• Hazard statements: H315-H319-H332-H360D
• Precautionary statements: P201-P261-P264-P280-P304+P340+P312-P308+P313
• Hazard Codes: Xn,Xi
• Risk Statements: 22-68/20/21/22-20/21/22-36-36/37/38-41
• Safety Statements: 36-45-36/37-26-39
• WGK Germany: 3
• RTECS: XF8045000
• F: 8-10-23
• TSCA: Yes
• HS Code: 32041900
• Toxicity: LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990