Home Categories Chemical Reagents Nitrotetrazolium Blue chloride
A6156612

Nitrotetrazolium Blue chloride , 98% , 298-83-9

Synonym(s):
NBT;Nitrotetrazolium Blue chloride;Nitroblue tetrazolium chloride;NBT, Nitro BT;Nitro BT

CAS NO.:298-83-9

Empirical Formula: C40H30ClN10O6+

Molecular Weight: 782.19

MDL number: MFCD00012159

EINECS: 206-067-4

Pack Size Price Stock Quantity
100MG RMB47.20 In Stock
500MG RMB88.80 In Stock
1G RMB160.00 In Stock
5G RMB492.00 In Stock
25g RMB1593.60 In Stock
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Update time: 2022-07-08

PRODUCT Properties

Melting point: 200°C
Density  1.5521 (rough estimate)
refractive index  1.7350 (estimate)
storage temp.  2-8°C
solubility  methanol: 50 mg/mL, clear, very strongly yellow
form  Powder/Solid
color  Yellow
Water Solubility  Soluble in water, ethanol and methanol.
Sensitive  Light Sensitive
λmax 256 nm
BRN  4115923
Stability: Stable, but store cold.
Biological Applications Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting

Description and Uses

Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.

Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.

Safety

Symbol(GHS) 
GHS07,GHS08
Signal word  Danger
Hazard statements  H315-H319-H332-H360D
Precautionary statements  P201-P261-P264-P280-P304+P340+P312-P308+P313
Hazard Codes  Xn,Xi
Risk Statements  22-68/20/21/22-20/21/22-36-36/37/38-41
Safety Statements  36-45-36/37-26-39
WGK Germany  3
RTECS  XF8045000
8-10-23
TSCA  Yes
HS Code  32041900
Toxicity LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990

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