4-Methylumbelliferyl β-D-glucopyranoside , 99% , 18997-57-4

Synonym(s):
MUD

CAS NO.：18997-57-4

Empirical Formula: C16H18O8

Molecular Weight: 338.31

MDL number: MFCD00063694

EINECS: 242-736-7

PRODUCT Properties

• Melting point: 258-263 °C (dec.)
• Boiling point: 394.46°C (rough estimate)
• Density: 1.522
• refractive index: 1.4430 (estimate)
• storage temp.: Sealed in dry,Store in freezer, under -20°C
• solubility: DMF: 5 mg/ml; DMSO: 50 mg/ml
• pka: 12.73±0.70(Predicted)
• form: Powder
• color: White to off-white
• Water Solubility: Soluble in DMF or water
• λmax: 320nm(Buffer)(lit.)
• BRN: 94672
• CAS DataBase Reference: 18997-57-4(CAS DataBase Reference)

Description and Uses

4-Methylumbelliferyl-β-D-Glucopyranoside (4-MUG) is a fluorogenic substrate of β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase). In addition to its use in characterizing novel β-glucosidases, 4-MUG is used in assays to evaluate deficiency in β- glucocerebrosidase activity related to Gaucher disease. Hydrolysis of 4-MUG releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.

4-Methylumbelliferyl β-D-glucopyranoside has been used as substrate:

• in glucosylceramidase β enzyme activity assay in lysosome-enriched fractions from primary hippocampal neurons
• in β-glucosidase assay during yeast fermentation
• to assay glucocerebrosidase 1 (GBA1)-related glucosidase activity in macrophage cell line (RAW)

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H302-H315-H319-H335
• Precautionary statements: P261-P280-P301+P312-P302+P352-P305+P351+P338
• Hazard Codes: T+
• Risk Statements: 26/27/28
• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 3-10-21
• HS Code: 29389090