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A4716312

HEPES , ≥99.5%(T) , 7365-45-9

Synonym(s):
HEPES;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid;HEPES solution;N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid);N-2-Hydroxyethylpiperazine-N?-2-ethanesulfonic Acid

CAS NO.:7365-45-9

Empirical Formula: C8H18N2O4S

Molecular Weight: 238.3

MDL number: MFCD00006158

EINECS: 230-907-9

Pack Size Price Stock Quantity
25G RMB49.60 In Stock
100G RMB145.60 In Stock
500G RMB517.60 In Stock
1KG RMB976.80 In Stock
5KG RMB3668.80 In Stock
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Update time: 2022-07-08

PRODUCT Properties

Melting point: 234-238 °C
Boiling point: 408℃[at 101 325 Pa]
Density  1.07 g/mL at 20 °C
vapor pressure  0Pa at 25℃
refractive index  n20/D 1.339
storage temp.  2-8°C
solubility  H2O: 1 M at 20 °C, clear, colorless
form  powder
color  White
PH 5.0-6.5 (1Maqueous solution)
pka 7.5(at 25℃)
PH Range 7.0 - 7.6
Water Solubility  Soluble
λmax λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
Merck  14,4654
BRN  883043
Stability: Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
InChIKey JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP -3.85 at 20℃

Description and Uses

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

Safety

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319-H335
Precautionary statements  P261-P271-P280
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  24/25-22-36-26
WGK Germany  1
RTECS  TL6809000
TSCA  Yes
HS Code  29335995

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