G 418 disulfate salt , potency:≥650μgpermg , 108321-42-2

Synonym(s):
;, NMD Inhibitor I;neomycin;NMD Inhibitor I

CAS NO.：108321-42-2

Empirical Formula: C20H42N4O14S

Molecular Weight: 594.63

MDL number: MFCD05664725

EINECS: 600-864-4

PRODUCT Properties

• storage temp.: 2-8°C
• solubility: H₂O: 50 mg/mL
• form: white powder
• color: white to off-white
• optical activity: [α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC))
• Water Solubility: soluble
• Stability: Hygroscopic
• InChIKey: QNQZPJLBGRQFDD-ZLRXUJDMSA-N

Description and Uses

Geneticin (also known as G418, G418 sulfate) is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. Geneticin blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells and is commonly used in laboratory research to select genetically engineered cells. Resistance to Geneticin is conferred by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APH 3'II.

Geneticin is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL. It is also used to membrane-permeant allosteric effector of hemoglobin therapeutic in cardiovascular diseases and cancer.

Safety

• Symbol(GHS): GHS08
• Signal word: Danger
• Hazard statements: H317-H334
• Precautionary statements: P261-P272-P280-P284-P302+P352-P333+P313
• Hazard Codes: Xn,Xi
• Risk Statements: 20/21/22-36/37/38-42/43
• Safety Statements: 22-24/25-36/37-23-36-26-45
• WGK Germany: 3
• RTECS: CB9378500
• HS Code: 29419090