Fluorescein diacetate , 97% , 596-09-8
Synonym(s):
3,6-Diacetoxyfluoran;Di-O-acetylfluorescein;Fluorescein diacetate;Fluorescein Diacetate - CAS 596-09-8 - Calbiochem
CAS NO.：596-09-8
Empirical Formula: C24H16O7
Molecular Weight: 416.38
MDL number: MFCD00005062
EINECS: 209-877-6
| Pack Size | Price | Stock | Quantity |
| 1g | RMB45.60 | In Stock |
|
| 5G | RMB176.80 | In Stock |
|
| 25G | RMB606.40 | In Stock |
|
| 100G | RMB2173.60 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | 200-203 °C(lit.) |
| Boiling point: | 494.73°C (rough estimate) |
| Density | 1.2738 (rough estimate) |
| refractive index | 1.6810 (estimate) |
| storage temp. | −20°C |
| solubility | acetone: soluble25mg/mL |
| form | Crystalline Powder |
| pka | 6.4(at 25℃) |
| color | Light yellow |
| PH Range | Weak green uorescence (6.0) to strong green uorescence (7.2) |
| Water Solubility | Soluble in chloroform, DMSO, ethanol, and water. |
| λmax | 490nm |
| BRN | 62575 |
| Major Application | LED devices, petroleum markers, identifying cancerous cells, detecting bacteria, virus, mitochondrial function in neurons, target molecules, living cells, viable Giardia cysts in water samples, apoptosis, evaluating live/dead state of algae, measurement of totalmicrobial activity in soil, treating ischemia |
| Biological Applications | Assessing membrane
integrity/permeability;43–50 detecting/counting microorganisms; measuring microbial activity;
as a substrate for measuring acylases activity,
amidases activity,aminopeptidases activity,
arylsulfatases activity,chymotrypsin activity,
esterases activity;galactosidases activity,
glucosidases activity, lipases activity,
phosphatases activity, proteases activity; cell viability
assay; cytotoxicity assay; implantable
medical devices; as temperature sensor; treating
ischemic disease |
| CAS DataBase Reference | 596-09-8(CAS DataBase Reference) |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)- (596-09-8) |
Description and Uses
Fluorescein diacetate (FDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, this AM ester yields fluorescein.
Fluorescein diacetate is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. It has been used for the differentiation of live cells from dead cells. It can be also used as a fluorogenic substrate for hGSTP1-1.
Safety
| Symbol(GHS) |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315-H319 |
| Precautionary statements | P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 24/25-36/37/39-27-26 |
| WGK Germany | 3 |
| TSCA | Yes |
| HS Code | 32129000 |
