Home Categories Biochemical Engineering Ethidium bromide(EB)
A3964112

Ethidium bromide(EB) , ≥95%(HPLC) , 1239-45-8

Synonym(s):
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide;EtBr;Homidium bromide

CAS NO.:1239-45-8

Empirical Formula: C21H20BrN3

Molecular Weight: 394.31

MDL number: MFCD00011724

EINECS: 214-984-6

Pack Size Price Stock Quantity
1G RMB82.40 In Stock
5G RMB280.80 In Stock
25G RMB1289.60 In Stock
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Update time: 2022-07-08

PRODUCT Properties

Melting point: 260-262 °C (dec.)(lit.)
Density  1.3739 (rough estimate)
refractive index  1.6700 (estimate)
Flash point: >100°C
storage temp.  2-8°C
solubility  H2O: 10 mg/mL, opaque, strongly red
form  powder
color  Red to dark purple
Odor Odorless solid
Water Solubility  40 g/L (25 ºC)
λmax 518 nm, 210 nm, 285 nm, 316 nm, 343 nm, 480 nm, 525 nm
Merck  14,4731
BRN  3642536
Stability: Stable. Incompatible with strong oxidizing agents.
Biological Applications Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification
Major Application Electroluminescent displays;photoresists

Description and Uses

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).

Safety

Symbol(GHS) 
GHS06,GHS08
Signal word  Danger
Hazard statements  H331-H341
Precautionary statements  P202-P261-P271-P280-P304+P340+P311-P308+P313
Hazard Codes  T,T+
Risk Statements  23-68-36/37/38-26-21/22-22
Safety Statements  36/37-45-36/37/39-28A-26-22-28-63
RIDADR  UN 2811 6.1/PG 1
WGK Germany  3
RTECS  SF7950000
8-9
HazardClass  6.1
PackingGroup  I
HS Code  29339990
Toxicity Intercalating dye widely used to stain DNA in gels and gradients. The DNA can be visualized readily by irradiation with ultraviolet light, as little as 0.5 μg of DNA being detectable by such methods. Ethidium bromide is also added to cesium chloride density gradients, since as an intercalating molecule it binds more readily to linear DNA than to closed collinear circles of DNA (such as plasmids). Binding of ethidium bromide reduces the density of DNA, thus covalent circles of DNA have higher densities at saturating concentrations of ethidium bromide, permitting the separation of plasmid DNA. Ethidium bromide is a putative carcinogen.

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