Home Categories Analytical Chemistry o-Dianisidine dihydrochloride
A3203212

o-Dianisidine dihydrochloride , 98.5%, biochemical level , 20325-40-0

Synonym(s):
o-Dianisidine dihydrochloride;3,3′-Dimethoxybenzidine dihydrochloride;Fast Blue B

CAS NO.:20325-40-0

Empirical Formula: C14H17ClN2O2

Molecular Weight: 280.75

MDL number: MFCD00012488

EINECS: 243-737-5

Update time: 2022-07-08

PRODUCT Properties

Melting point: 268 °C
Flash point: 206℃
storage temp.  2-8°C
solubility  H2O: soluble25mg/mL
form  tablet
color  Light beige to purple-gray
PH 1.7 at 20℃ and 100g/L
Water Solubility  1-5 g/100 mL at 20 ºC
BRN  3917996
InChIKey UXTIAFYTYOEQHV-UHFFFAOYSA-N
CAS DataBase Reference 20325-40-0(CAS DataBase Reference)
EPA Substance Registry System 3,3'-Dimethoxybenzidine dihydrochloride (20325-40-0)

Description and Uses

o-Dianisidine (dihydrochloride) is a colorimetric peroxidase substrate that has been used as a substrate to semi-quantitatively measure lactate, uric acid , and glucose. It has also been used as a reagent for the detection of various metals, thiocyanates, and nitrites. This aromatic amine is initially colorless but turns to violet upon oxidization and is spectrophotometrically measured at a wavelength of 405 nm.

o-Dianisidine (3,3′-dimethoxybenzidine) has been used as a component of GO (glucose oxidase) reagent, which is used for measuring the glucose content. It has also been used as a substrate to measure myeloperoxidase activity.

Safety

Symbol(GHS) 
GHS07,GHS08
Signal word  Danger
Hazard statements  H302-H350
Precautionary statements  P202-P264-P270-P280-P301+P312-P308+P313
Hazard Codes  T
Risk Statements  45-22
Safety Statements  53-45
RIDADR  2811
WGK Germany  3
RTECS  DD1050000
TSCA  Yes
HazardClass  6.1(a)
PackingGroup  II
HS Code  29222990

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