Home Categories Chemical Reagents 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT)
A1613850

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT) , Super pure , 298-93-1

Synonym(s):
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue;Methylthiazolyldiphenyl-tetrazolium bromide;MTT;Thiazolyl blue, MTT

CAS NO.:298-93-1

Empirical Formula: C18H16BrN5S

Molecular Weight: 414.32

MDL number: MFCD00011964

EINECS: 206-069-5

Pack Size Price Stock Quantity
1g RMB271.20 In Stock
5g RMB919.20 In Stock
25g RMB3199.20 In Stock
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Update time: 2022-07-08

PRODUCT Properties

Melting point: 195 °C (dec.) (lit.)
Density  1.4886 (estimate)
storage temp.  2-8°C
solubility  methanol: 5 mg/mL, clear
form  powder
color  Yellow to orange
Water Solubility  Slightly soluble.Clear yellow solution at 5mg in 1ml of water on vortexing
λmax 378 nm, 242 nm
Sensitive  Light Sensitive
BRN  3825277
Stability: Hygroscopic
Biological Applications Cell viability assay; microbial growth assays; DNA quantification assays; tissue viability assays; detecting enzymes; measuring membrane potential; treating Alzheimer’s disease,asthma,cancer
InChIKey AZKSAVLVSZKNRD-UHFFFAOYSA-M
CAS DataBase Reference 298-93-1(CAS DataBase Reference)
EPA Substance Registry System 2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (298-93-1)

Description and Uses

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Safety

Symbol(GHS) 
GHS07,GHS08
Signal word  Warning
Hazard statements  H315-H319-H335-H341
Precautionary statements  P201-P202-P261-P302+P352-P305+P351+P338-P308+P313
Hazard Codes  Xi,T,Xn
Risk Statements  46-68-36/37/38
Safety Statements  22-24/25-53-45-36/37-26
WGK Germany  3
RTECS  XF8060000
3-8-10
TSCA  Yes
HS Code  29341000
Hazardous Substances Data 298-93-1(Hazardous Substances Data)

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