Aprotinin , High -level , 9087-70-1

Synonym(s):
Aprotinin from bovine lung;Trasylol;Trypsin inhibitor (basic);trypsin inhibitor, pancreas type (bpti);trypsin-kallikrein inhibitor

CAS NO.：9087-70-1

Empirical Formula: C284H432N84O78R2S7

Molecular Weight: 6495.44

MDL number: MFCD00130541

EINECS: 232-994-9

Pack Size	Price	Stock	Quantity
10mg	RMB543.20	In Stock
50mg	RMB2119.20	In Stock
others			Enquire

Update time: 2022-07-08

PRODUCT Properties

Melting point:	>234oC (dec.)
storage temp.	2-8°C
solubility	glycerol: soluble3mg/mL, clear, colorless (equilibration buffer containing 5% glycerol)
form	lyophilized powder
color	white
Water Solubility	Freely soluble in water and in aqueous buffers of low ionic strength.
Merck	13,761
Sequence	H-Pro-Asp-Phe-Cys-Leu-Glu-Pro-Pro-Tyr-Thr-Gly-Pro-Cys-Lys-Ala-Arg-Ile-Ile-Arg-Tyr-Phe-Tyr-Asn-Ala-Lys-Ala-Gly-Leu-Cys-Gln-Thr-Phe-Val-Tyr-Gly-Gly-Cys-Arg-Ala-Lys-Arg-Asn-Asn-Phe-Lys-Ser-Ala-Glu-Asp-Cys-Met-Arg-Thr-Cys-Gly-Gly-Ala-OH
Stability:	Stable. Incompatible with strong oxidizing agents.
InChIKey	RWLBZZCCAZDWSE-BNRYNCRGNA-N

Description and Uses

Aprotinin is a reversible inhibitor of serine proteases such as trypsin (K_i = 0.06 pM), chymotrypsin (K_i = 9.5 nM), and kallikrein (K_i = 0.8 nM). It is a much weaker inhibitor of thrombin (K_i = 0.1-0.8 mM) and trypsinogen (K_i = 2 μM). Aprotinin also competitively inhibits nNOS and iNOS with K_i values of 50 and 78 μM, respectively. Aprotinin is widely used as a protein purification tool to prevent proteases present in tissue samples from degrading the protein of interest.

A potent protease inhibitor.Aprotinin acts as a reversible serine protease blocking and a binding agent. It is widely employed as an inhibitor of trypsin. It is also used as a proteolytic inhibitor in radioimmunoassays of polypeptide hormones. It is involved in the purification of urokinase, trypsin, and chymotrypsin on immobilized aprotinin. Further, it is used in the quantification of kallikrein activity in mixtures of esterases and proteases.

Safety

Symbol(GHS)	GHS07
Signal word	Warning
Hazard statements	H315-H319-H335
Precautionary statements	P261-P271-P280
Hazard Codes	Xn,Xi
Risk Statements	42/43-36/37/38-20/21/22
Safety Statements	22-45-36/37-36-26
WGK Germany	1
RTECS	YN5080000
F	10-21
HS Code	35040000
Toxicity	LD50 i.v. in mice: 2500000 kallikrein inhibitor units/kg (Trautschold)