Home Categories Biochemical Engineering IPTG(isopropyl-β-D-thiogalactopyranoside)
A1598450

IPTG(isopropyl-β-D-thiogalactopyranoside) , ≥99.0%, super pure , 367-93-1

Synonym(s):
IPTG;Isopropyl-β-D-thiogalactopyranoside;IPTG solution;Isopropyl β-D-1-thiogalactopyranoside;Isopropyl β-D-thiogalactoside

CAS NO.:367-93-1

Empirical Formula: C9H18O5S

Molecular Weight: 238.3

MDL number: MFCD00063273

EINECS: 206-703-0

Pack Size Price Stock Quantity
1g RMB287.20 In Stock
5g RMB1199.20 In Stock
10g RMB2175.20 In Stock
25g RMB3999.20 In Stock
100g RMB9503.20 In Stock
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Update time: 2022-07-08

PRODUCT Properties

Melting point: 105 °C
alpha  -31 º (c=1, water)
Boiling point: 350.9°C (rough estimate)
Density  1.3329 (rough estimate)
refractive index  1.5060 (estimate)
storage temp.  2-8°C
solubility  Soluble in Water or Methanol.
form  Crystalline Powder
pka 13.00±0.70(Predicted)
color  White
Water Solubility  soluble
Merck  14,5082
BRN  4631
Stability: Stable. Incompatible with strong oxidizing agents.
InChIKey BPHPUYQFMNQIOC-NXRLNHOXSA-N

Description and Uses

IPTG (also known as Isopropyl-β-D-Thiogalactopyranoside) is a molecular biology reagent that functions as an inducer of galactosidase activity by binding to and inhibiting the repressor. It is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. IPTG cannot be hydrolyzed or broken down by the E. coli therefore the concentration remains constant during cell replication. IPTG is often used at a final concentration ranging from 0.5-1.0 mM. X-Gal is often used with IPTG since it provides an easy visual method to monitor whether protein expression has occurred. X-Gal as the name implies contains a galactose group that once metabolized causes a bright blue color change.

Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective β-galactosidase inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology.

Safety

Symbol(GHS) 
GHS07,GHS08
Signal word  Warning
Hazard statements  H319-H351
Precautionary statements  P281-P305+P351+P338
Hazard Codes  Xn,Xi
Risk Statements  19-40-66-36/37/38
Safety Statements  23-24/25-36/37-22-36/37/39-27-26
WGK Germany  3
10
TSCA  Yes
HS Code  29389090

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