8-Anilino-1-naphthalenesulfonic acid , 96% , 82-76-8

Synonym(s):
N-Phenyl peri acid;ANS;ANSA

CAS NO.：82-76-8

Empirical Formula: C16H13NO3S

Molecular Weight: 299.34

MDL number: MFCD00003998

EINECS: 201-438-7

PRODUCT Properties

• Melting point: 215-217 °C (dec.)(lit.)
• Density: 1.409±0.06 g/cm3(Predicted)
• storage temp.: Sealed in dry,Room Temperature
• solubility: DMF: soluble50mg/mL
• pka: 0.19±0.40(Predicted)
• form: Powder
• color: Dark green to brown to black
• Merck: 13,664
• BRN: 1843887
• InChI: InChI=1S/C16H13NO3S/c18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13/h1-11,17H,(H,18,19,20)
• InChIKey: FWEOQOXTVHGIFQ-UHFFFAOYSA-N
• SMILES: C1(S(O)(=O)=O)=C2C(C=CC=C2NC2=CC=CC=C2)=CC=C1
• CAS DataBase Reference: 82-76-8(CAS DataBase Reference)
• EPA Substance Registry System: 8-Anilino-1-naphthalenesulfonic acid (82-76-8)

Description and Uses

1,8-ANS is a fluorescent dye that binds with high affinity to hydrophobic surfaces of proteins. 1,8-ANS has an excitation maximum of 350 nm. It has an emission maximum of 520 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FABP2) it has emission maxima of 468-477 nm. 1,8-ANS binds to low polarity regions of protein surfaces making it well suited for determining the affinity of hydrophobic ligands to their corresponding binding proteins, such as the binding of free fatty acids to FABPs. 1,8-ANS binds to FABP2 with a K_d value of approximately 9.7 μM at 24.5°C.

Intermediate of dyestuff

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H315-H319-H335
• Precautionary statements: P261-P280a-P304+P340-P305+P351+P338-P405-P501a-P264-P280-P302+P352+P332+P313+P362+P364-P305+P351+P338+P337+P313
• Hazard Codes: Xi
• Risk Statements: 36-36/37/38
• Safety Statements: 26-36
• WGK Germany: -
• TSCA: Yes
• HS Code: 29214980