Agarose , Low melting point, suitable for separation of small nucleic acid clips , 9012-36-6

Synonym(s):
Agarose;Agarose, Beaded;Agarose, Type C - CAS 9012-36-6 - Calbiochem

CAS NO.：9012-36-6

Empirical Formula: C10H15N3O3

Molecular Weight:

MDL number: MFCD00081294

EINECS: 232-731-8

PRODUCT Properties

• Melting point: ≤90 °C (4% in water)(lit.)
• Boiling point: 100℃
• Density: 1.00 g/mL at 20 °C
• Flash point: 38 °C
• storage temp.: 2-8°C
• solubility: H₂O: 20 mg/mL hot, slightly hazy, very faintly yellow
• form: suspension (75% in water)
• color: White to slightly cream
• Odor: Odorless
• Water Solubility: Soluble in water.
• Sensitive: Moisture Sensitive & Hygroscopic
• Stability: Stable. Incompatible with strong acids, strong oxidizing agents.
• LogP: -4.988 (est)
• CAS DataBase Reference: 9012-36-6
• EPA Substance Registry System: Agarose (9012-36-6)

Description and Uses

Agarose is the neutral polymer component of agar. Agar belongs to the group of galactopolysaccharides, which are the intracellular matrix of red algae/seaweeds of the Rhodophyta (red algae phylum). Agar is naturally slightly sulphated and is a complex mixture of polysaccharides. Agarose forms a solution when heated and condenses into a gel when cooled. Gel formation is due to the formation of a double helix of molecular chains, which creates a water-fixing network. Agarose is commonly used for the analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern), and is also suitable for protein analyses such as Ouchterlony and Radial Immunodiffusion (RID). Ethidium bromide and SYBR Green background staining is low.

Agarose is frequently used in molecular biology for the separation of large molecules, especially DNA, by electrophoresis. Slabs of agarose gels (usually 0.7 - 2%) for electrophoresis are readily prepared by pouring the warm, liquid solution into a mold. A wide range of different agaroses, of varying molecular weights and properties are commercially available for this purpose. Used in high resolution protein electrophoresis, immunoelectrophoresis, electro immunoassays such as rocket assays, counter current electrophoresis.

Safety

• Symbol(GHS): GHS02
• Signal word: Warning
• Hazard statements: H226
• Precautionary statements: P210-P233-P303+P361+P353-P370+P378-P403+P235-P501
• Hazard Codes: Xi
• Risk Statements: 10-36/37/38
• Safety Statements: 16-26-36
• WGK Germany: 3
• F: 3
• TSCA: Yes
• HS Code: 39139000